pdhB [2019-08-01 08:53:33]

pyruvate dehydrogenase (E1 beta subunit)

locus: BSU_14590

pI: 4.55

mw: 35.32 kDa

protein length: 325 aa

gene length: 978 bp

function: links glycolysis and TCA cycle

product: pyruvate dehydrogenase (E1 beta subunit)

essential: no

ec: 1.2.4.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 1,529,445 1,530,422

Phenotypes of a mutant

• defects in sporulation and unable to grow on glucose as single carbon source [Pubmed|11976308]
• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• Pyruvate [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine CO₂ (according to Swiss-Prot)

Paralogous protein(s)

• [protein|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|AcoB], [protein|A024921961A786294199EA12E04456C890ED8D8C|BkdAB]

Kinetic information

• Michaelis-Menten [Pubmed|6414463]

Modification

• phosphorylation on (Ser-302 OR Ser-306) [Pubmed|17218307]

Effectors of protein activity

• Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
• Low sensibility to NADPH

Structure

• [PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'')

[SW|Localization]

• membrane associated [Pubmed|18763711]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
  description: [Pubmed|11976308]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]

  regulation

  • ''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]

Biological materials

Mutant

• GP459 (spc), available in [SW|Jörg Stülke]'s lab
• BKE14590 ([gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA
• BKK14590 ([gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA

lacZ fusion

• pGP722 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

References

Reviews

• 19476487,9655937,2227213,6805383,24798336

Original publications

• 9352926,15378759,12850135,17218307,18763711,6414463,11976308,28189581